TRF concentrations in various tissues following anterior hypothalamic lesions.

TRF(Thyrotrophin-Releasing-Factor) is produced in the anterior hypothalamus, deposited in the neurohypophysis, and then passed into the cerebrospinal fluid and the blood stream to be excreted into urine(Shibusawa et al., 1959c). It has been demonstrated neither in the median and posterior hypothalamus, nor in other tissues, and body fluids. It is an hitherto unknown hormone which controls the thyroid function by acting upon the adenohypophysis via the hypophyseal portal vein or systemic circulation(Shibusawa et al., 1959c). The concentration of this" hormone" in the anterior hypothalamus and the neurohypophysis varies chiefly depending on the balance between the production and release of TRF. The activity of the anterior hypothalamus, therefore, should not be discussed by the assay of the concentration of the hormone in these areas alone. In this experiment, the distribution of TRF was investigated in various tissues of dogs after giving electrolytic lesions to the anterior hypothalamus bilaterally, which is regarded as the site of the production of TRF.